DNA Barcoding

The genome of members of the genus of Tulipa is comparatively very large (about 32Gb), ten times larger than the human genome and is difficult to sequence.

A total of 43 plant samples have been collected, representing almost all Greek species of tulip. Genomic DNA has been successfully extracted and quantified using a CTAB protocol (large-scale DNA extraction) for all tulip samples to be used in the next phase of experiments.
Plant samples from each species of Greek tulip will be genetically characterized by molecular markers.

The DNA nucleotide sequences are analyzed and submitted to the World Gene Bank (GenBank) for each species, and a comparative phylogenetic diagram will be created based on the DNA sequences of the Greek tulip species in relation to bibliographic data.

Phases of DNA extraction and isolation from leaf tissue of Greek tulips